Repository of Research and Investigative Information

Repository of Research and Investigative Information

Rafsanjan University of Medical Sciences

Diminished circulating concentration of interleukin-35 in Helicobacter pylori-infected patients with peptic ulcer: Its association with FOXP3 gene polymorphism, bacterial virulence factor CagA, and gender of patients

(2018) Diminished circulating concentration of interleukin-35 in Helicobacter pylori-infected patients with peptic ulcer: Its association with FOXP3 gene polymorphism, bacterial virulence factor CagA, and gender of patients. Helicobacter. p. 11. ISSN 1083-4389

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Abstract

BackgroundIL-35 modulates immune and inflammatory responses during infections. Here, we investigated IL-35 levels and a single nucleotide polymorphism, rs3761548, in FOXP3 gene in Helicobacter pylori-infected patients with peptic ulcer (PU), to clarify possible associations. Materials and MethodsThis study includes 100 H.pylori-infected PU patients, 100 H.pylori-infected asymptomatic subjects (AS), and 100 noninfected healthy subjects (NHSs). Serum IL-35 levels and the genotyping were determined using ELISA and RFLP-PCR methods, respectively. ResultsIn PU patients, the IL-35 levels were lower than AS and NHS groups (P<.001). The IL-35 levels in CagA(+)H.pylori-infected participants from PU and AS groups were lower than individuals infected with CagA(-) strains (P<.02 and P<.04, respectively). Women had higher IL-35 levels than men among PU, AS, and NHS groups (P<.0001). In PU patients, AA genotype and A allele at rs3761548 were more frequent than total healthy subjects (AS + NHS groups) and associated with an increased PU risk (AA genotype: OR = 5.51, P<.0001; A allele: OR = 3.857, P<.002). In PU and AS groups, IL-35 levels were lower in subjects displaying AA genotype or A allele than subjects displaying CC genotype or C allele, respectively (P<.0001 and P<.03 for PU patients; P<.001 and P<.02 for AS group, respectively). ConclusionsDecreased IL-35 levels could be involved in PU development in H.pylori-infected individuals. IL-35 levels are affected by CagA status of H.pylori, participants gender, and genetic variations at rs3761548. The AA genotype and A allele at rs3761548 could represent a risk factor for PU development.

Item Type: Article
Keywords: FOXP3 Helicobacter pylori interleukin-35 peptic ulcer regulatory t-cells healthy-children gastric-cancer south-east il-35 serum expression disease inflammation iran Gastroenterology & Hepatology Microbiology
Page Range: p. 11
Journal or Publication Title: Helicobacter
Journal Index: ISI
Volume: 23
Number: 4
Identification Number: https://doi.org/10.1111/hel.12501
ISSN: 1083-4389
Depositing User: مهندس مهدی شریفی
URI: http://eprints.rums.ac.ir/id/eprint/4252

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