Repository of Research and Investigative Information

Repository of Research and Investigative Information

Rafsanjan University of Medical Sciences

Pretreatment with melatonin protects against cyclophosphamide-induced oxidative stress and renal damage in mice

(2017) Pretreatment with melatonin protects against cyclophosphamide-induced oxidative stress and renal damage in mice. Fundamental & Clinical Pharmacology. pp. 625-635. ISSN 0767-3981

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Cyclophosphamide (CP) is widely used in treatment of different cancers. Nephrotoxicity is one of the dose-limiting side effects of CP. This study was carried out to investigate the effect of melatonin (MEL) on CP-induced nephrotoxicity in mice. In this study, 50 Swiss albino mice (20-25g) were randomly divided into five groups. Mice were pretreated with MEL intraperitoneally (i.p) in doses of 5, 10 and 20mg/kg for five consecutive days, and CP (200mg/kg, i.p) was administrated on the 5th day 1h after the last dose of MEL. Then on day 6, blood samples were collected to determine serum creatinine (Cr) and blood urea nitrogen (BUN) levels. The kidneys were used for histological examination, biochemical assays and real-time PCR studies. Malondialdehyde (MDA), glutathione (GSH), protein carbonyl (PC), nitric oxide (NO) level, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and myeloperoxidase (MPO) activity were assessed in renal tissue. In addition, the expression of SOD2 and PGx1 was measured using real-time PCR method in renal tissue. Results showed that CP administration significantly increases Cr, BUN, MDA, PC, NO level and MPO activity. It also decreases renal GSH level, SOD, GPx and CAT activity. Pretreatment with MEL (especially 20mg/kg, i.p.) for 5days prevented these changes; however, it did not affect the SOD activity. Our results revealed that MEL might be useful for prevention of the nephrotoxicity induced by CP through ameliorative effects on biochemical indices and oxidative stress parameters.

Item Type: Article
Keywords: cyclophosphamide melatonin mice nephrotoxicity gentamicin-induced nephrotoxicity nitric-oxide rat-kidney injury antioxidant apoptosis ischemia reperfusion involvement mechanisms Pharmacology & Pharmacy
Page Range: pp. 625-635
Journal or Publication Title: Fundamental & Clinical Pharmacology
Journal Index: ISI
Volume: 31
Number: 6
Identification Number:
ISSN: 0767-3981
Depositing User: مهندس مهدی شریفی

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