Repository of Research and Investigative Information

Repository of Research and Investigative Information

Rafsanjan University of Medical Sciences

Down-regulation of anti-apoptotic genes in tumor cell lines is facilitated by suppression of OCT4B1

(2017) Down-regulation of anti-apoptotic genes in tumor cell lines is facilitated by suppression of OCT4B1. Advances in Medical Sciences. pp. 97-102. ISSN 1896-1126

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Abstract

Purpose: The OCT4B1 as a variant of OCT4 is expressed in both cancer cells and tissues. The antiapoptotic property of this variant aid cancer cells to escape from apoptosis. Therefore, the aim of the present study was to determine the effects of OCT4B1 suppression on regulation of 25 genes involved in anti-apoptotic pathway in tumor cell lines. Material and methods: AGS (gastric adenocarcinoma), 5637 (bladder tumor) and U-87MG (brain tumor) cells were transfected with specific OCT4B1 siRNA and a scramble siRNA by siRNA silencing gene technology, using Lipofectamine 2000 commercial kit. The real-time PCR technique was employed to examine and calculate fold changes of evaluated genes using the 2 (Delta Delta CT) formula. Results: Present results demonstrated that 22 (88) of interested genes were similarly down-regulated in all three examined cell lines. Our results also indicated that three genes (CASP2, IGF1R, TNF) were up-regulated. The CFLAR gene was down-regulated in AGS, while it was inversely up-regulated in 5637 and U87MG cells. Conclusions: It may possibly be concluded that suppression of OCT4B1 can lead to apoptosis in tumor cell lines and this is at least facilitated via down-regulation of examined anti-apoptotic genes. Accordingly, suppression of OCT4B1 may probably be considered as useful tool in cancer therapy and research. (C) 2016 Medical University of Bialystok. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.

Item Type: Article
Keywords: OCT4B1 Apoptosis Gene Tumor Cell lines cancer stem-cells spliced variant self-renewal expression protein involvement carcinoma family death oct-4 Research & Experimental Medicine
Page Range: pp. 97-102
Journal or Publication Title: Advances in Medical Sciences
Journal Index: ISI
Volume: 62
Number: 1
Identification Number: https://doi.org/10.1016/j.advms.2016.04.004
ISSN: 1896-1126
Depositing User: مهندس مهدی شریفی
URI: http://eprints.rums.ac.ir/id/eprint/4402

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