Repository of Research and Investigative Information

Repository of Research and Investigative Information

Rafsanjan University of Medical Sciences

Ginger extract modulates the expression of IL-12 and TGF-beta in the central nervous system and serum of mice with experimental autoimmune encephalomyelitis

(2017) Ginger extract modulates the expression of IL-12 and TGF-beta in the central nervous system and serum of mice with experimental autoimmune encephalomyelitis. Avicenna Journal of Phytomedicine. pp. 54-65. ISSN 2228-7930

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Abstract

Objective: The main function of IL-12 is differentiation of naive T cells intoTh1 cells and TGF-beta is a powerful immunoregulatory cytokine. The immunomodulatory and anti-inflammatory properties of ginger have also been reported in some studies. The aim of this study was to evaluate the effects of ginger extract on the expression of IL-12 and TGF-beta in a model of experimental autoimmune encephalomyelitis (EAE). Materials and Methods: EAE was induced in C57BL/6 mice by immunization with myelin oligodendroglial glycoprotein emulsified in complete Freund's adjuvant. The mice were administered intraperitoneally with ginger extracts or PBS, from day +3 to +30. On day 31, mice were scarified and the expression of IL-12 and TGF-beta mRNA in the spinal cord were determined by using real time-PCR. The serum levels of cytokines were measured by ELISA. Results: In PBS-treated EAE mice, the expression of IL-12 P35 and IL-12 P40 mRNA in the CNS and the mean serum levels of IL-12 were significantly higher than those of healthy group (p<0.001). In ginger-treated EAE mice, the expression of IL-12 mRNA and its serum levels were significantly lower as compared to PBS-treated EAE mice. No significant difference was observed between PBS-treated EAE mice and healthy group regarding the expression of TGF-beta mRNA. In ginger (300 mg/kg)-treated EAE group, the expression of TGF-beta mRNA and its serum levels were significantly higher in comparison to PBS-treated EAE mice (p< 0.01 and p<0.05, respectively). Conclusion: These results indicated that ginger extract modulates the expression of IL-12 and TGF-beta in CNS and serum of EAE mice.

Item Type: Article
Keywords: Experimental autoimmune encephalomyelitis Ginger IL-12 TGF-beta Serum multiple-sclerosis evaluation circulating levels gene polymorphism immune tolerance t-cells disease activation model inflammation macrophages Pharmacology & Pharmacy
Page Range: pp. 54-65
Journal or Publication Title: Avicenna Journal of Phytomedicine
Journal Index: ISI
Volume: 7
Number: 1
ISSN: 2228-7930
Depositing User: مهندس مهدی شریفی
URI: http://eprints.rums.ac.ir/id/eprint/4439

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