Repository of Research and Investigative Information

Repository of Research and Investigative Information

Rafsanjan University of Medical Sciences

In vitro and in silico studies of the interaction of three tetrazoloquinazoline derivatives with DNA and BSA and their cytotoxicity activities against MCF-7, HT-29 and DPSC cell lines

(2017) In vitro and in silico studies of the interaction of three tetrazoloquinazoline derivatives with DNA and BSA and their cytotoxicity activities against MCF-7, HT-29 and DPSC cell lines. International Journal of Biological Macromolecules. pp. 85-95. ISSN 0141-8130

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Abstract

In this study, the interaction of three 1,2,3,4tetrazolo5,1-6-quinazolin-8-one derivatives with salmon sperm DNA and BSA was investigated experimentally and theoretically. Fluorescence and absorption spectroscopy techniques were applied to determine the probable interaction mechanism and correlated binding constants and thermodynamic parameters. It was found that the compounds intercalate into the DNA duplex via minor groove in a moderately strong fashion with the binding constants of 10(4)M(-1). The values of binding constant for the interaction with BSA at different temperatures were also calculated to be in the range of 1.06 x 10(3)-3.54 x 10(8) M-1 indicating the relatively high propensity of the compounds to BSA. In vitro cytotoxicity studies on the effect of the compounds on MCF-7 and HT-29 cancerous cell lines as well as DPSC normal cell line were performed using MIT assay. It was observed that the compounds exhibited selective inhibitory effect against cancer cell growth. Moreover, each of the title compounds was separately docked to DNA and BSA using Autodock Vina as a molecular docking program. The obtained theoretical results were in good agreement with those obtained from Experimental studies. (C) 2016 Elsevier B.V. All rights reserved.

Item Type: Article
Keywords: BSA and DNA interaction Molecular docking Spectroscopic techniques MTT assays 1,2,3,4tetrazolo5,1-b-quinazolin-8-one derivatives human serum-albumin molecular docking copper(ii) complexes anticancer activities spectroscopic methods crystal-structure binding-site cleavage agents intercalators Biochemistry & Molecular Biology Chemistry Polymer Science
Page Range: pp. 85-95
Journal or Publication Title: International Journal of Biological Macromolecules
Journal Index: ISI
Volume: 94
Identification Number: https://doi.org/10.1016/j.ijbiomac.2016.09.113
ISSN: 0141-8130
Depositing User: مهندس مهدی شریفی
URI: http://eprints.rums.ac.ir/id/eprint/4441

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