Repository of Research and Investigative Information

Repository of Research and Investigative Information

Rafsanjan University of Medical Sciences

Possible involvement of nitrergic and opioidergic systems in the modulatory effect of acute chloroquine treatment on pentylenetetrazol induced convulsions in mice

(2016) Possible involvement of nitrergic and opioidergic systems in the modulatory effect of acute chloroquine treatment on pentylenetetrazol induced convulsions in mice. Brain Research Bulletin. pp. 124-130. ISSN 0361-9230

Full text not available from this repository.

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

Chloroquine has long been used for the treatment of malaria and rheumatological disorders. Accumulating evidence suggests potential use of chloroquine as a neuroprotective agent. Several studies have reported that endogenous opioids and nitric oxide (NO) system mediate the chloroquine effects. In the present study, the involvements of endogenous opioids and NO in the modulatory effects of chloroquine on pentylenetetrazol-induced seizures were assessed in mice. Chloroquine 5 mg/kg significantly increased the seizure threshold, but this effect was reversed with naltrexone 1 mg/kg. Acute co-administration of L-NAME (non-selective NO synthase (NOS) inhibitor, 5 mg/kg) or 7-NI (selective neuronal NOS inhibitor, 40 mg/kg) with the effective dose of chloroquine completely inhibited its anticonvulsant effects. Acute single injection of a sub-effective dose of L-arginine (NO precursor, 60 mg/kg) with a sub-effective dose of chloroquine 2.5 mg/kg increased the seizure threshold but administration of L-arginine 60 mg/kg with chloroquine 10 mg/kg decreased the seizure threshold. Moreover, the combination of the lower doses of naltrexone (0.1 mg/kg) and 7-NI (15 mg/kg) showed additive effects in blocking the chloroquine-induced anticonvulsant properties. Chloroquine 5 mg/kg enhanced the hippocampal nitrite levels. Chloroquine at the dose of 20 mg/kg decreased the seizure threshold. This effect was inhibited through L-NAME (5 mg/kg), 7-NI (40 mg/kg) and naltrexone (1 mg/kg) administration with this dose of chloroquine. In conclusion, NO signaling probably through neuronal NOS, but not inducible NOS could be involved in the opioid-dependent anticonvulsant effects of chloroquine in this model of seizures in mice. It seems that nitric oxide and opioid systems are involved in modulatory effect of chloroquine on seizures induced by pentylenetetrazol. (C) 2016 Published by Elsevier Inc.

Item Type: Article
Keywords: Chloroquine Opioid Nitric oxide Pentylenetetrazole Clonic seizure threshold Mice nitric-oxide synthase induced seizure threshold antiepileptic drugs endogenous opioids epileptic seizures up-regulation morphine cells rats inhibitor Neurosciences & Neurology
Page Range: pp. 124-130
Journal or Publication Title: Brain Research Bulletin
Journal Index: ISI
Volume: 121
Identification Number: https://doi.org/10.1016/j.brainresbull.2015.11.020
ISSN: 0361-9230
Depositing User: مهندس مهدی شریفی
URI: http://eprints.rums.ac.ir/id/eprint/4503

Actions (login required)

View Item View Item