Repository of Research and Investigative Information

Repository of Research and Investigative Information

Rafsanjan University of Medical Sciences

Increased mRNA Level of Orexin1 and 2 Receptors Following Induction of Experimental Autoimmune Encephalomyelitis in Mice

(2016) Increased mRNA Level of Orexin1 and 2 Receptors Following Induction of Experimental Autoimmune Encephalomyelitis in Mice. Iranian Journal of Allergy Asthma and Immunology. pp. 20-26. ISSN 1735-1502

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Abstract

Orexin A and B are hypothalamic peptides with a wide variety of effects such as antiinflammation and neuroprotection. Impaired function of orexin system has been reported in some neurodegenerative diseases like Parkinson, Huntington and Alzheimer. In this study, the mRNA expression levels of some hypothalamic peptides were investigated in C57BL/6 female mice with experimental autoimmune encephalomyelitis (EAE). Animals were randomly divided into two control and EAE groups. EAE was induced by administration of myelin oligodendrocyte glycoprotein (MOG) with complete Ferund's adjuvant and pertussis toxin. Twenty-first days following immunization, mice were decapitated to remove the brains. Then, the expression profiles of prepro-orexin, orexin 1 receptors (OX1R) and orexin 2 receptors (OX2R) in hypothalamic region were assessed using real-time PCR method. In this study, we found a considerable increase in the mRNA expression of OX1R and OX2R following EAE induction in C57BL/6 mice. Elevation levels of OX1R and OX2R following EAE induction suggest that alteration in orexinergic system may involve in pathogenesis of multiple sclerosis.

Item Type: Article
Keywords: C57BL/6 mice Experimental autoimmune encephalomyelitis Multiple sclerosis Orexin 1 receptors Orexin 2 receptors Prepro-orexin multiple-sclerosis cerebrospinal-fluid hypocretin-1 disease neurons orexin/hypocretin degeneration hypersomnia sleepiness fatigue Allergy Immunology
Page Range: pp. 20-26
Journal or Publication Title: Iranian Journal of Allergy Asthma and Immunology
Journal Index: ISI
Volume: 15
Number: 1
ISSN: 1735-1502
Depositing User: مهندس مهدی شریفی
URI: http://eprints.rums.ac.ir/id/eprint/4510

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