Repository of Research and Investigative Information

Repository of Research and Investigative Information

Rafsanjan University of Medical Sciences

Opium induces apoptosis in Jurkat cells via promotion of pro-apoptotic and inhibition of anti-apoptotic molecules

(2016) Opium induces apoptosis in Jurkat cells via promotion of pro-apoptotic and inhibition of anti-apoptotic molecules. Iranian Journal of Basic Medical Sciences. pp. 215-220. ISSN 2008-3866

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Abstract

Objective(s): The aim of this study was to determine the important molecules involved in apoptosis induction by opium in Jurkat cell line. Materials and Methods: Jurkat cells were incubated 48 hrs with 2.86x10(-5) g/ml concentration of opium and apoptosis as well as expression levels of related molecules were measured. Results: Our results demonstrated that 50.3 +/- 0.2 percent of opium treated Jurkat cells were revealed apoptotic features. The levels of mRNA of several pro-apoptotic and anti-apoptotic molecules were increased and decreased, respectively, in the opium treated cells. The results also demonstrated that expression levels of BCL2, DFFA and NOL3 as anti-apoptotic molecules were increased in the opium treated cells. Conclusion: It seems that opium induces apoptosis in Jurkat cells via both intrinsic and extrinsic pathways. Although opium induces apoptosis in the cells but increased expression of some antiapoptotic molecules may be a normal resistance of the cell for death.

Item Type: Article
Keywords: Apoptosis Jurkat cells PCR array tumor-necrosis-factor expression profiles Research & Experimental Medicine Pharmacology & Pharmacy
Page Range: pp. 215-220
Journal or Publication Title: Iranian Journal of Basic Medical Sciences
Journal Index: ISI
Volume: 19
Number: 2
ISSN: 2008-3866
Depositing User: مهندس مهدی شریفی
URI: http://eprints.rums.ac.ir/id/eprint/4513

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