Repository of Research and Investigative Information

Repository of Research and Investigative Information

Rafsanjan University of Medical Sciences

Inhibitory Effects of Silver Nanoparticles on Growth and Aflatoxin B-1 Production by Aspergillus Parasiticus

(2015) Inhibitory Effects of Silver Nanoparticles on Growth and Aflatoxin B-1 Production by Aspergillus Parasiticus. Iranian Journal of Medical Sciences. pp. 501-506. ISSN 0253-0716

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Background: Aflatoxins (AFs) are secondary hazardous fungal metabolites that are produced by strains of some Aspergillus species on food and feedstuffs. Aflatoxin B-1 (AFB(1)) is one of the most important AF with high toxicity. Prevention of AF production and their elimination from food products is a matter of importance for many researchers in the last decades. Nanomaterials applications in medical science have been widely studied in the recent years. Most of existing researches seek the effect of nanoparticles on bacteria, fungi, and viruses. The aim of this study was to determine the effects of silver nanoparticles (AgNPs) on growth and AFB(1) production of AF-producing Aspergillus parasiticus. Methods: A parasiticus was inoculated (10(6) conidia per ml of medium) to potato dextrose broth (PDB) medium and then AgNPs was added and incubated with shaking at 130 rpm and 28 degrees C for 7 days. AF was assayed by high performance liquid chromatography (HPLC). Microbiological assay (MBA) on microplates contained potato dextrose broth (PDB) medium (4 days at 28 degrees C) at different concentrations of AgNPs (60, 80, 100, 120, 140, 160, 180 and 200 mu g/ml) was measured. Results: The results demonstrated that a minimum inhibition concentration (MIC) equal to 180 mu g/ml was determined for AgNPs against A. parasiticus. The AgNPs effectively inhibited AFB(1) production at a concentration of 90 mu g/ml. Conclusion: The results obtained in this study show AgNPs at concentrations lower than the MIC drastically inhibited production of AFB(1) by A. parasiticus in culture medium. The AgNPs may be useful to control AF contamination of susceptible crops in the field.

Item Type: Article
Keywords: Nanoparticles Aflatoxins Aspergillus parasiticus High pressure Liquid chromatography General & Internal Medicine
Page Range: pp. 501-506
Journal or Publication Title: Iranian Journal of Medical Sciences
Journal Index: ISI
Volume: 40
Number: 6
ISSN: 0253-0716
Depositing User: مهندس مهدی شریفی

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