Repository of Research and Investigative Information

Repository of Research and Investigative Information

Rafsanjan University of Medical Sciences

Vitamin D Modulates the Expression of IL-27 and IL-33 in the Central Nervous System in Experimental Autoimmune Encephalomyelitis

(2015) Vitamin D Modulates the Expression of IL-27 and IL-33 in the Central Nervous System in Experimental Autoimmune Encephalomyelitis. Iranian Journal of Immunology. pp. 35-49. ISSN 1735-1383

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Abstract

Background: It has been reported that vitamin D has broad anti-inflammatory and immunomodulatory effects. Objective: To evaluate the effects of vitamin D on the expression of IL-27 and IL-33 in a model of experimental autoimmune encephalomyelitis (EAE). Methods: EAE was induced in C57BL/6 mice by immunization with myelin oligodendroglial glycoprotein mixed with complete Freund's adjuvant. The mice were administered with PBS or olive oil, intraperitoneally, in the control groups and vitamin D (200 ng every two days) in the treatment group, from day + 3 to + 30. At day 31, the mice were scarified and their spinal cords and brains were harvested. The expression of the IL-27 and IL-33 mRNA in the spinal cord was measured using real time-PCR. Results: In PBS- or olive oil-treated EAE mice the expression of IL-27 P28 mRNA was significantly lower than that in the healthy control group (p < 0.002). In both PBS-and olive oil-treated EAE groups, the expression of IL-27 EBI3 mRNA was also lower than that observed in the healthy group, but the differences were not significant. In vitamin D-treated EAE group, the expression of IL-27 P28 and IL-27 EBI3 were significantly higher compared with the olive oil-treated EAE groups (p < 0.002 and p < 0.04, respectively). The expression of IL-33 was significantly higher in PBS- or olive oil-treated EAE groups compared with healthy mice (p < 0.05 and p < 0.02, respectively). Vitamin D significantly decreased the expression of IL-33 compared with PBS- or olive oil-treated EAE mice (p < 0.04, p < 0.02, respectively). The PBS-or e oiloliv-treated EAE mice showed the clinical symptoms of EAE at days 9 and 10, respectively. The vitamin D-treated EAE group exhibited the symptoms at day 12 post immunization. The maximum mean clinical score and mean pathological scores were also significantly lower in vitamin D-treated EAE group, in comparison with PBS-or olive oil treated EAE mice (p < 0.001). Conclusion: Vitamin D may modulate the expression of IL-27 and IL-33 in the spinal cord of EAE mice and also ameliorate the clinical symptoms of the disease.

Item Type: Article
Keywords: Experimental Autoimmune Encephalomyelitis IL-27 IL-33 Vitamin D multiple-sclerosis patients cd4(+) t-cells ifn-gamma family-members interleukin-33 mechanisms induction secretion cytokines disease Immunology
Page Range: pp. 35-49
Journal or Publication Title: Iranian Journal of Immunology
Journal Index: ISI
Volume: 12
Number: 1
ISSN: 1735-1383
Depositing User: مهندس مهدی شریفی
URI: http://eprints.rums.ac.ir/id/eprint/4586

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