Repository of Research and Investigative Information

Repository of Research and Investigative Information

Rafsanjan University of Medical Sciences

Decreased Expressions of Toll-Like Receptor 9 and Its Signaling Molecules in Chronic Hepatitis B Virus-Infected Patients

(2013) Decreased Expressions of Toll-Like Receptor 9 and Its Signaling Molecules in Chronic Hepatitis B Virus-Infected Patients. Archives of Pathology & Laboratory Medicine. pp. 1674-1679. ISSN 0003-9985

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Abstract

Context.-Toll-like receptors (TLRs) play crucial roles in immune responses, especially innate immunity, against viral infections. Toll-like receptor 9 recognizes intracellular viral double-strand DNA, which leads to the activation of nuclear factor B (NF-kappa B) through the myeloid differentiation primary response 88 (MYD88) pathway. Defects in the expression of TLR9 and its signaling molecules may cause attenuated immune responses against hepatitis B virus. Objective.-To determine expression levels of TLR9 messenger RNA along with MYD88, interleukin 1 receptor-associated kinase 1 (IRAK1), tumor necrosis factor receptor-associated factor 6 (TRAF6), and NF-kappa B in the peripheral blood mononuclear cells obtained from chronic hepatitis B virus (CHB)-infected patients. Design.-In this study, 60 CHB patients and 60 healthy controls were recruited and the expression of TLR9 and its downstream signaling molecules was examined by real-time polymerase chain reaction techniques using beta-actin as a housekeeping gene. Results.-Our results showed that expression of TLR9, MYD88, IRAK1, TRAF6, and NF-kappa B in peripheral blood mononuclear cells of CHB patients was significantly decreased in comparison with healthy controls. Conclusions.-According to our results, it appears that CHB patients are unable to appropriately express genes in the TLR9 pathway, which may impede immune responses against hepatitis B virus infection. These results suggest a mechanism that may partially explain the fact that immune responses are disrupted in CHB patients.

Item Type: Article
Keywords: plasmacytoid dendritic cells blood mononuclear-cells hepatocellular-carcinoma hbv infection iranian patients activation genotype tlr9 gene Medical Laboratory Technology Research & Experimental Medicine Pathology
Page Range: pp. 1674-1679
Journal or Publication Title: Archives of Pathology & Laboratory Medicine
Journal Index: ISI
Volume: 137
Number: 11
Identification Number: https://doi.org/10.5858/arpa.2012-0415-OA
ISSN: 0003-9985
Depositing User: مهندس مهدی شریفی
URI: http://eprints.rums.ac.ir/id/eprint/4682

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