Repository of Research and Investigative Information

Repository of Research and Investigative Information

Rafsanjan University of Medical Sciences

Expression of Gro (CXCL1) and SDF-1 alpha (CXCL12) is differentially controlled by various intracellular signaling pathways in rat hepatocytes

(2012) Expression of Gro (CXCL1) and SDF-1 alpha (CXCL12) is differentially controlled by various intracellular signaling pathways in rat hepatocytes. African Journal of Pharmacy and Pharmacology. pp. 368-375. ISSN 1996-0816

Full text not available from this repository.

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

Chemokines play key roles in physiological and pathological activities of liver diseases. This study aimed to examine the intracellular signaling pathways involved in regulation of expression of Gro (CXCL1) and SDF-1 alpha (CXCL12). Hepatocytes were isolated from rat liver and were cultured on collagen Type-I. Initially, cells were suspended in inoculation medium and then were added to the pre-wetted plates. Then the cells were treated with specified concentrations of inhibitor and incubated in 5 CO2: 95 O-2 for 3 h. Medium was removed, centrifuged and separated using SDS-PAGE. The immunoblotting was used to analyze chemokines expression. The results demonstrated that at lower concentrations of SB203580, no detectable inhibition of Gro (CXCL1) and SDF-1 alpha was observed, while there was a significant decrease in its expression at higher concentrations. Gro (CXCL1) was only decreased in the presence of the highest concentration of MG132 and the expression of SDF-1 alpha (CXCL12) was not inhibited by MG132. KN62 inhibited expression of Gro (CXCL1) at higher concentrations but had no effect on SDF-1 alpha (CXCL12) expression. Analysis of data showed that Staurosporine produces a significant decrease in expression of it, but Gro (CXCL1) was only inhibited at 10 mu M concentration of Staurosporine. These data showed that SDF-1 alpha (CXCL12) and Gro (CXCL1) are expressed following hepatocyte isolation and can be regulated by the inhibitors of p38, NF-KB, CAMK-II and staurosporine. Furthermore, there may be further potential to prevent changes to hepatocyte phenotype and allow isolation of hepatocytes with a greater physiological phenotype.

Item Type: Article
Keywords: Chemokine hepatocyte CXCL12 CXCL1 kappa-b activation p38 map kinase gene-expression chemokine expression murine hepatocytes epithelial-cells heat-shock factor-i cytokine liver Pharmacology & Pharmacy
Page Range: pp. 368-375
Journal or Publication Title: African Journal of Pharmacy and Pharmacology
Journal Index: ISI
Volume: 6
Number: 6
Identification Number: https://doi.org/10.5897/ajpp11.284
ISSN: 1996-0816
Depositing User: مهندس مهدی شریفی
URI: http://eprints.rums.ac.ir/id/eprint/4776

Actions (login required)

View Item View Item