Repository of Research and Investigative Information

Repository of Research and Investigative Information

Rafsanjan University of Medical Sciences

T Helper and B Cell Escape Mutations within the HBc Gene in Patients with Asymptomatic HBV Infection: A Study From the South-Eastern Region of Iran

(2012) T Helper and B Cell Escape Mutations within the HBc Gene in Patients with Asymptomatic HBV Infection: A Study From the South-Eastern Region of Iran. Clinical Laboratory. pp. 53-60. ISSN 1433-6510

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Abstract

Background: Escape mutations potentially allow viruses to avoid detection and clearance by the host immune system and may represent a mechanism through which infections may persist in some patients. The association of the mutations in the HBcAg gene with Hepatitis B asymptomatic carriers (ASC) has not been studied adequately. The current study was aimed to investigate HBcAg18-27 CTL epitope mutations in ASC patients in the South-Eastern region of Iran. Methods: 100 ASC patients were selected for this study and screened for HLA-A2 using flow cytometry. HBV-DNA was extracted from the HLA-A2 positive patients and the HBc gene was amplified using PCR. Direct double sequencing was performed to analyse mutations in the HBc gene of HBV isolates from patients with ASC. Results: Overall, 25 (25) of individuals were HLA-A2 positive. Direct double sequencing indicated no mutations in the HBcAg18-27 epitope. However, four mutations within the T helper and three mutations within the B cell epitopes of ASC patients were identified. Conclusions: The lack of mutations within the HBcAg18-27 epitope suggests that the antigenicity of this region is not altered in HBV isolates of our patients and therefore antigen presentation would occur normally to the patient's immune system through HLA-A2. However, in the course of this study we revealed some novel mutations within the T helper and B cell epitopes that may affect the efficiencies of immune response of ASC patients against these novel HBV epitopes. (Clin. Lab. 2012;58:53-60)

Item Type: Article
Keywords: Asymptomatic HBV infection HLA-A2 HBcAg mutation hepatitis-b core antigen virus protein genotype epitope DNA Medical Laboratory Technology
Page Range: pp. 53-60
Journal or Publication Title: Clinical Laboratory
Journal Index: ISI
Volume: 58
Number: 1-2
ISSN: 1433-6510
Depositing User: مهندس مهدی شریفی
URI: http://eprints.rums.ac.ir/id/eprint/4789

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