Repository of Research and Investigative Information

Repository of Research and Investigative Information

Rafsanjan University of Medical Sciences

Expression of stromal derived factor alpha (SDF-1 alpha) by primary hepatocytes following isolation and heat shock stimulation

(2008) Expression of stromal derived factor alpha (SDF-1 alpha) by primary hepatocytes following isolation and heat shock stimulation. Iranian Journal of Allergy Asthma and Immunology. pp. 61-68. ISSN 1735-1502

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Abstract

Stromal derived factor-alpha (SDF-1-alpha) is a CXC chemokine which has been demonstrated as a recruitment factor for leukocytes to the site of inflammation, infection, injury and following stress. This chemokine has been shown to be expressed by liver cells and in liver diseases. Hence, the aim of this study was to examine the expression of SDF-1 by hepatocytes in responses to the stress Imposed during isolation by collagenase perfusion and under heat shock Stimulation. In this study hepatocytes (2-5 x 10(6)) were isolated from male Sprague Dawley rat liver and Cultured in plates that were pre-coated with collagen Type-I matrix. The western and northern blotting analysis were employed to detect SDF-1 at protein and mRNA levels in isolated and cultured hepatocytes in response to isolation and heat shock stresses. The SDF-1 is expressed by isolated rat hepatocytes immediately after isolation and early culture and decreased with time. SDF-1 protein was highly expressed in freshly isolated cells and decreased by time (27h) (P<0.05). mRNA was also expressed in freshly isolated cells (0h) but decreased after 24h of culture (P<0.01). This results also demonstrated that expression of SDF-1 by hepatocytes was increased in response to heat shock at different time points comparing with control (P<0.01). These results demonstrated that the isolation and heat shock stresses induced the expression of SDF-1 in hepatocytes in a time-dependent manner. Accordingly, it seems that hepatocytes mimic the experiences that fiver experience after injury in vivo and therefore, produce stress related agents like chemokines to overcome such a injurious condition.

Item Type: Article
Keywords: chemokine hepatocyte liver SDF-1 alpha cxc chemokine pbsf/sdf-1 b-cell lymphopoiesis epithelial-cells differential expression liver gene hepatoma injury kc Allergy Immunology
Page Range: pp. 61-68
Journal or Publication Title: Iranian Journal of Allergy Asthma and Immunology
Journal Index: ISI
Volume: 7
Number: 2
ISSN: 1735-1502
Depositing User: مهندس مهدی شریفی
URI: http://eprints.rums.ac.ir/id/eprint/4909

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