Repository of Research and Investigative Information

Repository of Research and Investigative Information

Rafsanjan University of Medical Sciences

Evaluation of effects of methamphetamine repeated dosing on proliferation and apoptosis of rat germ cells

(2008) Evaluation of effects of methamphetamine repeated dosing on proliferation and apoptosis of rat germ cells. Systems Biology in Reproductive Medicine. pp. 85-91. ISSN 1939-6368

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Methamphetamine (MAMP) is a central nervous system stimulant that is increasingly abused especially by teenagers and young adults, a group in its reproductive age. MAMP effects on the male reproductive system are not clear. In this experimental study, we evaluated the effects of MAMP administration on proliferation and apoptosis in seminiferous tubules of rat testis. Methamphetamine hydrochloride was synthesized by iodination of norephedrine hydrochloride and reduction to rnethamphetamine. Mature male rats were randomly divided into four groups (n = 7) and were injected intraperitoneally with MAMP (1, 5 or 10 mg/kg) or saline at the same time, once daily for 14 consecutive days. Twenty four hs after the last injection, perfused testis were fixed, sectioned and stained by TUNEL labeling or proliferating cell nuclear antigen (PCNA) immunostaining. Apoptotis and proliferation indices were calculated and ratios of proliferation/apoptosis in the seminiferous tubules were obtained. Cell proliferation and the ratio of proliferation to apoptosis decreased significantly in all experimental groups compared to the control group. Conversely, apoptosis was increased in these groups. Such differences were observed in both spermatogonia and primary spermatocytes. In the control group, more than 95 of spermatogonia were PCNA-positive. However, 10 mg/kg of MAMP caused a reduction to approximately 75 PCNA staining in spermatogonia. In some tubules of the experimental groups, more than 10 TUNEL-positive germ cells were seen, although in the control group, the tubules with 3 TUNEL-positive germ cells were rarely observed and the majority of tubules were without such cells. There were significant differences in the indices between the 1 mg/kg group and the higher dose groups but there was no such difference between the 5 mg/kg and 10 mg/kg groups In. some tubules of the experimental groups, significant gaps in. the epithelium between the spermatogonia layer and other cell layers were observed. These results show that repeated administration of MAMP, especially at higher doses, may cause a decrease in cellular proliferation, induce apoptosis and change the proliferation/apoptosis ratio in testis. This might explain the MAMP effect on the spermatogenesis process. It is suggested that studies on die consequence of MAMP consumption on male fertility is warranted.

Item Type: Article
Keywords: apoptosis male reproductive system methamphetamine proliferating cell nuclear antigen proliferation rat spermatogenesis amphetamines fixation exposure ecstasy testis Endocrinology & Metabolism Reproductive Biology
Page Range: pp. 85-91
Journal or Publication Title: Systems Biology in Reproductive Medicine
Journal Index: ISI
Volume: 54
Number: 2
Identification Number:
ISSN: 1939-6368
Depositing User: مهندس مهدی شریفی

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