Repository of Research and Investigative Information

Repository of Research and Investigative Information

Rafsanjan University of Medical Sciences

Low dose revaccination induces robust protective anti-HBs antibody response in the majority of healthy non-responder neonates

(2008) Low dose revaccination induces robust protective anti-HBs antibody response in the majority of healthy non-responder neonates. Vaccine. pp. 269-276. ISSN 0264-410X

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A sizeable proportion (1-10) of healthy adults and to lesser extent neonates vaccinated with triple 10 mu g hepatitis B (HB) vaccine fail to mount a protective antibody response. Revaccination with the same vaccine dose has proved to be effective in a significant number of primary non-responders. The influence of revaccination with lower vaccine doses however has not been studied adequately in non-responder neonates. This study was conducted to evaluate the influence of supplementary vaccination with a single low and standard dose of a recombinant hepatitis B (HB) vaccine in healthy Iranian non-responder neonates to primary vaccination. Iranian neonates unable to respond to primary vaccination with 10, 5 or 2.5 mu g doses of recombinant HB vaccine were revaccinated with a single additional dose of the same concentration. Serum anti-HBs antibody titer was measured by sandwich ELISA. Administration of a single additional dose induced seroprotection (anti-HBs >= 10IU/L) in 10/12 (83), 10/12 (83) and 21/24 (87.5) of non-responder neonates in 10, 5 and 2.5 l,g vaccine recipients with geometric mean titers (and 95 confidence limits) of 1358 (258-7142), 401 (79-2038) and 164 (62-433) IU/L, respectively. The log-transformed antibody titer obtained for the 10 mu g dose recipients was significantly higher than that of the 2.5 mu g dose vaccinees (p = 0.028). No significant differences in anti-HBs titer were observed between other groups of vaccinees. However, the total seroprotection rates obtained after administration of four low doses of 2.5 and 5 mu g were significantly higher than that obtained after administration of the classical three 10 mu g doses (p = 0.029 and p = 0.006, respectively). The total seroprotection rates were similar between all groups of vaccines receiving four doses of 2.5, 5 and 10 mu g vaccine doses. These results indicate that a significant proportion of non-responder neonates can be induced to develop a protective anti-HBs response following revaccination with a single low dose vaccine. Thus adaptation of four low dose (2.5 or 5 mu g) vaccination is expected to induce higher seroprotection rate and lower or comparable anti-HBs antibody titer in healthy neonates. (c) 2007 Elsevier Ltd. All rights reserved.

Item Type: Article
Keywords: hepatitis B vaccine anti-HBs antibody revaccination non-responder neonates protective response hepatitis-b-vaccine th2 cytokine production surface-antigen immunological memory immune-response follow-up recombinant immunization virus cells Immunology Research & Experimental Medicine
Page Range: pp. 269-276
Journal or Publication Title: Vaccine
Journal Index: ISI
Volume: 26
Number: 2
Identification Number:
ISSN: 0264-410X
Depositing User: مهندس مهدی شریفی

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