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Rafsanjan University of Medical Sciences

Leishmania major: Genetic heterogeneity of Iranian isolates by single-strand conformation polymorphism and sequence analysis of ribosomal DNA internal transcribed spacer

(2006) Leishmania major: Genetic heterogeneity of Iranian isolates by single-strand conformation polymorphism and sequence analysis of ribosomal DNA internal transcribed spacer. Acta Tropica. pp. 52-58. ISSN 0001-706X

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Abstract

Protozoan parasites of Leishmania major are the causative agents Of Cutaneous leishmaniasis in different parts of Iran. We applied PCR-based methods to analyze L. major parasites isolated from patients with active lesions from different geographic areas in Iran in order to understand DNA polymorphisms with in L. major species. Twenty-four isolates were identified as L. major by RFLP analysis of the ribosomal internal transcribed spacer I (ITS1) amplicons. These isolates were further Studied by single-strand conformation polymorphism (SSCP) analysis and sequencing of ITS1 and ITS2. Data obtained from SSCP analysis of the ITS1 and ITS2 loci revealed three and four different patterns among all studied samples, respectively. Sequencing of ITS1 and ITS2 confirmed the results of SSCP analysis and showed the potential of the PCR-SSCP method for assessing genetic heterogeneity within L. major. Different patterns in ITS1 were due to substitution of one nucleotide, whereas in ITS2 the changes were defined by variation in the number of repeats in two polymorphic microsatellites. In total five genotypic groups LmA, LmB, LmC, LmD and LmE were identified among L. major isolates. The most frequent genotype, LmA, was detected in isolates collected from different endemic areas of cutaneous leishmaniasis in Iran. Genotypes LmC, LmD and LmE were found only in the new focus of CL in Damghan (Semnan province) and LmB was identified exclusively among isolates of Kashan focus (Isfahan province). The distribution of genetic polymorphisms suggests the existence of distinct endemic regions of L. major in Iran. (c) 2006 Elsevier B.V. All rights reserved.

Item Type: Article
Keywords: Leishmania major genetic analysis ITS 1-RFLP PCR-SSCP internal transcribed spacer cutaneous leishmaniasis clinical-samples molecular epidemiology infantum donovani identification variability taxonomy disease Parasitology Tropical Medicine
Page Range: pp. 52-58
Journal or Publication Title: Acta Tropica
Journal Index: ISI
Volume: 98
Number: 1
Identification Number: https://doi.org/10.1016/j.actatropica.2006.01.010
ISSN: 0001-706X
Depositing User: مهندس مهدی شریفی
URI: http://eprints.rums.ac.ir/id/eprint/4933

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