Repository of Research and Investigative Information

Repository of Research and Investigative Information

Rafsanjan University of Medical Sciences

The antibody response to HBs antigen is regulated by coordinated Th1 and Th2 cytokine production in healthy neonates

(2003) The antibody response to HBs antigen is regulated by coordinated Th1 and Th2 cytokine production in healthy neonates. Clinical and Experimental Immunology. pp. 451-456. ISSN 0009-9104

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Abstract

A proportion of healthy neonates fail to produce protective levels of anti-HBs antibody following vaccination with recombinant hepatitis B vaccine. This study was undertaken to investigate contribution of Th1 and Th2 responses to anti-HBs antibody production and to explore the mechanism(s) of unresponsiveness to HBsAg in human neonates. Peripheral blood manonuclear cells (PBMCs) were isolated form 28 nonresponder (anti-HBs antibody <10 IU/l) and 25 responder neonates. The cells were stimulated in vitro with recombinant HBsAg and PHA mitogen and concentrations of IL-4, IL-10 and IFN-gamma were quantified in culture supernatants by sandwich ELISA. Our results demonstrated significantly increased production of all cytokines, including IL-4 (P < 0.001), IL-10 (P < 0.002) and IFN-gamma (P < 0.01) in responder compared to nonresponder vaccinees. No significant differences, however, were observed between the two groups of neonates in the levels of cytokines induced by PHA or secreted in absence of antigen and mitogen. Our findings suggest that unresponsiveness to recombinant HBsAg in healthy neonates is linked to inadequate secretion of both Th1 and Th2 cytokines.

Item Type: Article
Keywords: hepatitis B vaccination neonates anti-HBs antibody Th1/Th2 cytokines hepatitis-b-vaccine surface-antigen dendritic cells t-cells nonresponders lymphocytes specificity tolerance infection humans Immunology
Page Range: pp. 451-456
Journal or Publication Title: Clinical and Experimental Immunology
Journal Index: ISI
Volume: 131
Number: 3
Identification Number: https://doi.org/10.1046/j.1365-2249.2003.02093.x
ISSN: 0009-9104
Depositing User: مهندس مهدی شریفی
URI: http://eprints.rums.ac.ir/id/eprint/4941

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