Repository of Research and Investigative Information

Repository of Research and Investigative Information

Rafsanjan University of Medical Sciences

The Modulatory Effects of Vitamin D on the Expression of IL-12 and TGF-beta in the Spinal Cord and Serum of Mice with Experimental Autoimmune Encephalomyelitis

(2018) The Modulatory Effects of Vitamin D on the Expression of IL-12 and TGF-beta in the Spinal Cord and Serum of Mice with Experimental Autoimmune Encephalomyelitis. Iranian journal of pathology. pp. 10-22. ISSN 1735-5303 (Print) 1735-5303 (Linking)

[img] Text
ijp-13-010.pdf

Download (691kB)

Official URL: https://www.ncbi.nlm.nih.gov/pubmed/29731791

Abstract

Background & objective: The immunoregulatory effects of transforming growth factor (TGF)-betaand interleukin-12 (IL-12) and immunomodulatory actions of vitamin D (VD) were reported in several studies. This study aims to evaluate VD effects on IL-12 and TGF-beta expression in experimental autoimmune encephalomyelitis (EAE). Methods: EAE was induced in three groups of C57BL/6 mice by immunization with MOG and administered intra-peritoneally 200 ngVD, PBS or olive oil (OO) from day +3 to +30. One group was also considered as healthy control group. At day 31, cytokines expression in the spinal cord and their serum levels were determined using real time-PCR and ELISA, respectively. Results: IL-12 gene expression and its serum levels in PBS-injected- or OO-administrated EAE groups were significantly higher than healthy group. IL-12 gene expression in EAE group treated with VD was significantly decreased compared to PBS-injected- or OO-administrated EAE groups (P<0.001 and P<0.02, respectively). Serum IL-12 levels were significantly reduced in VD-treated EAE mice compared to PBS-injected- or OO-administrated EAE groups (P<0.004 and P<0.05, respectively). No significant difference was observed between PBS-injected-EAE, OO-administrated EAE and healthy groups regarding the expression of TGF-beta. In EAE mice treated with VD, TGF-beta expression was significantly higher than healthy group and PBS-injected EAE mice (P<0.01 and P<0.04, respectively). VD-treated EAE group had also higher serum TGF-beta levels than PBS-injected-, OO-administrated and healthy groups (P<0.05). Conclusion: VD modulates the expression of IL-12 and TGF-beta in spinal cord and serum of EAE mice.

Item Type: Article
Keywords: Experimental Autoimmune Encephalomyelitis Il-12 TGF-beta Vitamin D
Page Range: pp. 10-22
Journal or Publication Title: Iranian journal of pathology
Journal Index: Pubmed
Volume: 13
Number: 1
ISSN: 1735-5303 (Print) 1735-5303 (Linking)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.rums.ac.ir/id/eprint/4978

Actions (login required)

View Item View Item