Repository of Research and Investigative Information

Repository of Research and Investigative Information

Rafsanjan University of Medical Sciences

Non-association of IL-12 +1188 and IFN-γ +874 polymorphisms with cytokines serum level in occult HBV infected patients

(2011) Non-association of IL-12 +1188 and IFN-γ +874 polymorphisms with cytokines serum level in occult HBV infected patients. Saudi Journal of Gastroenterology. pp. 30-35. ISSN 13193767 (ISSN)

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Abstract

Background/Aim: Occult hepatitis B infection (OBI) is identified as a form of hepatitis in which despite the absence of detectable HBsAg, HBV-DNA is observed in peripheral blood of patients. The main aim of this study has been to investigate the association between polymorphisms in +874 of IFN-γ and +1188 of IL-12 with their serum level in patients suffering from OBI. Materials and Methods: In this experimental study, plasma samples of 3700 blood donors were tested for the presence of hepatitis B surface antigen (HBsAg) and anti-HBc by ELISA. The HBsAg- /anti-HBc+ samples were selected and screened for HBV-DNA by PCR. HBV-DNA positive samples were assigned as OBI cases and ARMS-PCR techniques were performed to examine the two known polymorphisms within IL-12 and IFN-γ. In addition, the serum levels of IL-12 and IFN-γ were also determined by ELISA. Results: Results of this study demonstrated that, 352 (9.5) out of 3700 blood samples were HBsAg -/anti-HBc+ and HBV-DNA was detected in 57/352 (16.1) of HBsAg-/anti-HBc+ samples. Our results showed that groups showed significant difference in CC allele of +1188 region of IL-12 and no difference was observed in the other evaluated genes. Our results also showed that the alleles of +1188 region of IL-12 and alleles of +874 of IFN-γ were also not associated with serum level of cytokines. Conclusion: According to the results of this study, it may be concluded that the polymorphisms in +1188 region of IL-12 and +874 region of IFN-γ would not affect the expression of both cytokines at serum level in OBI patients.

Item Type: Article
Keywords: IFN-γ IL-12 occult hepatitis B infection polymorphism gamma interferon hepatitis B surface antigen interleukin 12 adult allele article blood donor blood sampling controlled study DNA polymorphism enzyme linked immunosorbent assay female genetic association hepatitis B human major clinical study male screening Case-Control Studies Hepatitis B Surface Antigens Humans Immunity, Cellular Interferon-gamma Interleukin-12 Polymorphism, Genetic Young Adult
Page Range: pp. 30-35
Journal or Publication Title: Saudi Journal of Gastroenterology
Journal Index: Scopus
Volume: 17
Number: 1
Identification Number: https://doi.org/10.4103/1319-3767.74461
ISSN: 13193767 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.rums.ac.ir/id/eprint/5355

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